Biosynthetic Gene Cluster of ad-Tryptophan-Containing Lasso Peptide, MS-271

MS 271.qxp

Study Objectives: The obstructive sleep apnea hypopnea syndrome (OSAHS) is a disorder characterized by repetitive closure and reopening of the upper airway during sleep. Upper airway luminal patency is influenced by the surface tension (γ) acting within the liquid layer lining the upper airway. The aim of the present study was to examine the γ of upper airway mucosal lining liquid (UAL) in both...

A pH-sensitive peptide-containing lasso molecular switch.

The synthesis of a peptide-containing lasso molecular switch by a self-entanglement strategy is described. The interlocked rotaxane molecular machine consists of a benzometaphenylene[25]crown-8 (BMP25C8) macrocycle surrounding a molecular axle. This molecular axle contains a tripeptidic sequence and two molecular stations: a N-benzyltriazolium and a pH-sensitive anilinium station. The tripeptid...

The tryptophan biosynthetic pathway

The trp repressor proteins regulate transcription of several different operons located at separate sites on the E. coli chromosome. In addition to the trp operon, this repressor represses transcription of aroH, a single-gene operon that encodes an enzyme required for the synthesis of all aromatic amino acids, and trpR, another single-gene operon encoding the trp repressor itself. Such coordinat...

Minimum Information about a Biosynthetic Gene cluster.

Genome mining unearths a hybrid nonribosomal peptide synthetase-like-pteridine synthase biosynthetic gene cluster

Nonribosomal peptides represent a large class of metabolites with pharmaceutical relevance. Pteridines, such as pterins, folates, and flavins, are heterocyclic metabolites that often serve as redox-active cofactors. The biosynthetic machineries for construction of these distinct classes of small molecules operate independently in the cell. Here, we discovered an unprecedented nonribosomal pepti...